Download e-book for kindle: Advanced Photon Counting: Applications, Methods, by Peter Kapusta,Michael Wahl,Rainer Erdmann

By Peter Kapusta,Michael Wahl,Rainer Erdmann

This quantity makes a speciality of Time-Correlated unmarried Photon Counting (TCSPC), a robust device permitting luminescence lifetime measurements to be made with excessive temporal solution, even on unmarried molecules. Combining spectrum and lifelong presents a “fingerprint” for selecting such molecules within the presence of a history. Used including confocal detection, this enables single-molecule spectroscopy and microscopy as well as ensemble measurements, establishing up a massive variety of sizzling existence technological know-how functions akin to fluorescence lifetime imaging (FLIM) and size of Förster Resonant power move (FRET) for the research of protein folding and interplay. a number of technology-related chapters current either the fundamentals and present cutting-edge, specifically of TCSPC electronics, photon detectors and lasers. the rest chapters conceal a vast diversity of purposes and methodologies for experiments and knowledge research, together with the existence sciences, disorder facilities in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new concepts bobbing up from the mix of vintage TCSPC and fluorescence lifetime with tools in accordance with depth fluctuation characterize a very detailed highlight.

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Advanced Photon Counting: Applications, Methods, Instrumentation (Springer Series on Fluorescence) by Peter Kapusta,Michael Wahl,Rainer Erdmann

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